IgM anti-GQ1b monoclonal antibody inhibits voltage-dependent calcium current in cerebellar granule cells

Miller–Fisher syndrome (MFS), which is known to be associated with anti-GQ1b antibodies and to cause ataxia, is a variant of an acute inflammatory neuropathy. However, the pathogenic role of anti-GQ1b antibodies remains unclear. In this study, we investigated the effects of mouse IgM anti-GQ1b monoclonal antibody (IgM anti-GQ1b mAb) on the spontaneous muscle action potential of a rat spinal cord-muscle co-culture system and on the voltage-dependent calcium channel (VDCC) current in cerebellar granule cells and Purkinje cells using the whole-cell patch clamp technique. The frequency of spontaneous muscle action potential of the innervated muscle cells was transiently increased by IgM anti-GQ1b mAb and then was blocked completely, which was the same finding as reported previously. Moreover, the cerebellar granule cell VDCC current was decreased by 30.76 ± 7.60% by 5 μg/mL IgM anti-GQ1b mAb, whereas IgM anti-GQ1b mAb did not affect the VDCC current in cerebellar Purkinje cells. In immunocytochemistry, IgM anti-GQ1b mAb stained the whole cell surface of cerebellar granule cells, but not that of Purkinje cells. Therefore, the clinical symptoms of Miller–Fisher syndrome, such as cerebellar-like ataxia, may be explained by the inhibitory effects of anti-GQ1b antibodies on VDCC current in cerebellar granule cells.     

Multiple activation currents can be evoked inXenopus laevis eggs when cortical granule exocytosis is inhibited by weak bases

The fertilization potential inXenopus eggs under normal circumstances is considered to be a unique event. It is associated with a concomitantly occurring cortical granule exocytosis. If eggs were exposed to weak bases, exocytosis was inhibited but the fertilization potential could still be evoked. After recovery from this first transient increase in membrane conductance, a second could be elicited by a further stimulus. A fertilization potential could be triggered either before or after the egg had undergone an electrically induced activation potential. This suggests that sperm receptors and sperm activated ionic channels in the egg membrane remain functional following the conductance change, at least when the exocytotic event was prevented.A transient conductance increase could only be induced by NH ₄ ⁺ (pH 9.0) in unactivated eggs that had not undergone cortical granule exocytosis.Tremendous variation was noticed between successive activation currents elicited in the same egg. Under voltageclamp at 0 mV holding potential, the current often changed from inward to outward. Although cortical granule exocytosis may only play a minor role in the transient conductance change triggered at fertilization, it may well be involved in subsequent modifications of membrane conductance.     

柏枣宁心颗粒治疗顽固性失眠症疗效观察

笔者自2004年1月至2008年1月，根据辨证论治以自拟柏枣宁心颗粒治疗顽固性失眠症，取得了较好疗效。现将结果报道如下。     

固本喘嗽康颗粒平喘、抗炎及免疫作用实验研究

目的:观察固本喘嗽康颗粒的平喘、抗炎及免疫作用。方法:用卵蛋白雾化吸入致敏法建立大鼠哮喘模型,观察固本喘嗽康颗粒对大鼠引喘潜伏期和BALF中白细胞数的影响;用二甲苯及琼脂建立炎症模型观察固本喘嗽康颗粒对急性炎症及慢性炎症的作用;从体液免疫和细胞免疫两方面观察固本喘嗽康颗粒的免疫作用。结果:固本喘嗽康颗粒可明显延长大鼠引喘潜伏期,降低BALF中白细胞数;可明显对抗急性炎症和慢性炎症;对体液免疫和细胞免疫具有调节作用。结论:固本喘嗽康颗粒具有平喘、抗炎和免疫调节的作用。     

灵芝配方颗粒中麦角甾醇的含量测定

目的:建立灵芝配方颗粒中麦角甾醇的含量测定方法。方法:反相HPLC,流动为甲醇-水(99∶1),检测波长为281 nm,流速1.2 mL·min-1,柱温25℃。结果:麦角甾醇对照品进样量在0.223 7～1.342μg呈良好线性关系,回归方程为Y=1 104.468X+1.168,r=0.999 9,回收率为103.1%,RSD为0.48%(n=6)。结论:本法简便易行,重复性好,可作为灵芝配方颗粒的质量控制方法。     

抗纤软肝颗粒对肝星状细胞细胞外信号调节激酶1mRNA、蛋白表达的影响

目的:探讨抗纤软肝颗粒对肝星状细胞细胞外信号调节激酶1(ERK1)mRNA、蛋白表达的影响。方法:运用细胞培养技术,以hsc-t6细胞系为研究对象,分为(1)空白包被+10%生理盐水组、(2)纤维连接蛋白(FN)+10%生理盐水组、(3)FN+5%抗纤软肝颗粒含药血清(kxr)组、(4)FN+10%kxr组、(5)FN+20%kxr组。按以上分组进行药物干预,干预后继续培养48h。应用免疫细胞组织化学方法检测ERK1蛋白的表达,采用RT-PCR法测定ERK1mRNA的表达。结果:与FN组相比,kxr组肝星状细胞的ERK1mRNA、蛋白表达均显著下调,分别减少了54.36%(35.98%)、66.08%(49.59%)、72.82%(59.15%),均有显著性差异。结论:kxr能够在转录及翻译水平抑制ERK1mRNA、蛋白的表达,从而阻抑了整合素激活的MAPK途径的信号转导。     

稳心颗粒联合美托洛尔治疗冠心病心律失常疗效观察

目的:观察稳心颗粒联合美托洛尔治疗冠心病心律失常的疗效。方法:治疗组60例口服稳心颗粒和美托洛尔治疗,对照组60例口服美托洛尔治疗。结果:临床症状疗效治疗组88.3%、对照组65.0%,动态心电图疗效治疗组86.7%、对照组61.7%,治疗组疗效优于对照组(P0.01)。结论:稳心颗粒联合美托洛尔治疗冠心病心律失常效果较好,且不良反应小。     

肠吉泰治疗腹泻型肠易激综合征的随机双盲安慰剂平行对照试验

目的观察中药验方肠吉泰治疗腹泻型肠易激综合征（肝郁脾虚型）的疗效和安全性。方法采用随机双盲、安慰剂平行对照的方法,将60例符合纳入标准的腹泻型肠易激综合征患者,以1：1比例按随机数字进入试验组或对照组（各30例）,对受试者、干预措施实施者及结果测量者实施盲法;试验组予肠吉泰颗粒剂,对照组予安慰剂,两组临床试验疗程均为28天;实施干预措施前、干预结束后对受试者采用症状尺度量表（IBS-BSS）、大便性状量表（IBS-DSQ）观察临床症状变化及生活质量改善情况,疗程结束后评价临床疗效。结果试验组和对照组主要症状有效率符合方案集（PP）分别为83.33%、62.10%,全分析集（FAS）分别为83.33%、60.00%（P〈0.05）;试验组和对照组的次要症状有效率符合方案集（PP）分别为86.67%、58.62%,全分析集（FAS）分别为86.67%、56.67%。组间主要症状和次要症状有效率比较,各分析集差异均有统计学意义（P〈0.05）。试验组治疗前后主要症状总积分及各单项症状积分差异均有统计学意义（P〈0.05）;对照组除腹胀症状外,主要症状总积分及其他各单项积分差异有统计学意义（P〈0.05）;组间治疗后比较,主要症状总积分及各单项积分差异均有统计学意义（P〈0.05）。两组治疗前后自身比较,排便次数和10天内大便急迫天数积分差异有统计学意义（P〈0.05）;组间治疗后比较,10天内大便急迫天数积分差异有统计学意义（P〈0.05）。两组治疗过程中均未发生不良事件。结论中药验方肠吉泰治疗腹泻型肠易激综合征（肝郁脾虚型）安全有效。     

复方肠泰抑制人结肠癌SW480细胞增殖的实验研究

目的:考察复方肠泰(人参、薏苡仁、莪术等)对人结肠癌细胞SW480细胞生长的抑制作用及其对细胞增殖周期和凋亡的影响。方法:采用噻唑蓝比色法测定不同浓度复方肠泰在不同作用时间内对在体外培养SW480细胞增殖的影响,同时应用流式细胞术检测细胞增殖周期及凋亡率的变化。结果:复方肠泰可在G0/G1期以及G2/M期阻滞人结肠癌细胞SW480的增殖,使S期细胞比率降低。结论:复方肠泰在3.51～7.81mg/mL范围内呈现明显的量效关系。     

降脂颗粒对四环素致脂肪肝小鼠血清ALT、AST及肝组织TG、TC水平的影响

目的：观察降脂颗粒对四环素致脂肪肝小鼠模型血清ALT、AST及肝组织TG、TC水平的影响。方法：将80只昆明种小鼠随机分为7组。各用药组给予相应药物,日1次,连续11d,从第8天起,除空白组外,各组小鼠均腹腔注射0.25mmol/kg四环素,共4d。末次给药后1h,处死小鼠,行生化和病理学检测。结果：①降脂颗粒能明显降低四环素致脂肪肝小鼠的肝脏指数,以降脂颗粒高剂量组为最好;②除模型组外,各用药组均可改善肝功能,以降脂颗粒高剂量组为最佳;③模型组小鼠肝脏TG、TC明显增高,各用药组均能降低肝组织TG、TC水平,以降脂颗粒高剂量组为最好。结论：降脂颗粒能明显降低由四环素所引起的小鼠血清ALT、AST及肝组织TG、TC水平的升高,改善脂肪变性,降低肝脏炎症反应,对四环素致脂肪肝小鼠的肝脏及炎症有较好的治疗作用。